In order to identify the genetic determinants that allow a root system to integrate and respond to its environment, we need to be able to visualize root development over time and systematically compare the root system architectures that are formed from different plant genotypes. GrassRoots is developing novel means of imaging and characterizing plant roots.
GrassRoots utilizes two complementary technologies to non-invasively image growing plant roots. The first is the Plant Root Visualization and Characterization System (PRVCS) developed by Phenotype Screening Corporation. The second system is a root growth and imaging system based on gellan gum. Both systems provide high-resolution images of growing roots. Dr. Benfey and collaborators have developed an image analysis pipeline utilizing both the PRVCS and gel-based images. These tools will provide quantitative measures of root architecture for a variety of parameters. Current efforts are directed at moving these from two-dimensions to three dimensions. We will employ these systems to optimize switchgrass roots for rapid root establishment and carbon sequestration.